Starch and protein recovery from brewer’s spent grain using hydrothermal pretreatment and their conversion to edible filamentous fungi – A brewery biorefinery concept

This study aimed at recovering a highly concentrated starch and protein stream from the brewer’s spent grain (BSG). The effect of pretreatment temperature retention time on solubilization protein; generation fermentation inhibitors were studied. Then, application recovered streams for fungal cultivation was evaluated using different edible fungi Aspergillus oryzae, Neurospora intermedia, Rhizopus delemar. hydrothermal resulted in highest solubilized concentration, 43 g/L, corresponding to 83% initial BSG content. concentration 27 g/L (48% content). Cultivation with intermedia two best conditions, 140 ? 4 h 180 30 min, pure biomass content 59.62% 50.42% w/w, respectively. Finally, brewery biorefinery proposed valorization BSG.